ABSTRACT
A precise, sensitive and quick High Performance Liquid Chromatographic [HPLC] method for the determination of rosuvastatin calcium in bulk and tablet dosage forms has been validated. The chromatographic scheme involved: Sil-20A auto sampler, LC-20A pump, SPD-20A UV/visible detector with separation attained by C18 column at 40oC temperature through a mobile phase of acetonitrile and buffer [50:50] at a flow rate of 1.0ml/min. The method is precise [%RSD for intra-day and inter-day extended between 1.06-1.54% and 0.103-1.78%] and linear [r[2]=0.9997]. Limit of detection and quantification [LOD and LOQ] of the adopted method were 0.78 and 1.56Mug/ml. The proposed HPLC method was established to be sensitive, precise and swift that can be proficiently adopted in quality control/quality assurance laboratories for predictable investigation of the bulk and oral solid dosage forms of rosuvastatin calcium
ABSTRACT
The objective of present study was to develop and evaluate polyethylene glycol [PEG] based diclofenac sodium suppositories. This study used water soluble PEG bases [1000, 4000 and 6000] in different combinations to formulate suppositories, which were further subjected for their physicochemical properties evaluation such as weight variation, average melting point, content uniformity and disintegration. Dissolution test was used to perform the in vitro release rate studies of the suppositories. The suppository [P3] containing PEG-6000 [50%] and PEG-4000 [50%] exhibited rapid in vitro release rate of diclofenac sodium. Moreover, homogeneous distribution of diclofenac sodium is found in all six formulations. The in vitro release patterns of diclofenac sodium from the marketed Voltral suppository [100mg] and formulated suppositories were also compared and found in standard limits
ABSTRACT
A Simple, sensitive and accurate high-performance liquid chromatographic [HPLC] method for effective and specific analysis of Loxoprofen [LXP] in the mobile phase and human plasma was developed. Effective chromatographic separation was attained on a Mediterranea Sea C18 column [250×4.6mm, 5um] with mobile phase containing acetonitrile and 0.01 M NaH2PO4 buffer [55: 45] by adjusting pH 6.5 with sodium dihydrogen phosphate buffer at a flow rate of 1ml/ min. Calibration ranges from 0.1ppm to 10 ppm with a coefficient of relation value [R2=0.999] by using a linear regression method and lower limit of quantification was 0.1ppm. The current method showed inter-day and intra-day accuracy and precision within the range of +/- 10%. % RSD was found to be less than 5 %. Analytical recovery was more than 90% which confirmed the reliability of current method. The proposed method was found appropriate for assessment of LXP in pharmacokinetic and bioequivalence study
ABSTRACT
In the present work a specific, accurate, precise, and reproducible UV-HPLC method was developed and validated for the analysis of Aceclofenac. This method involved elution of Aceclofenac in a mobile phase which is composed of buffer pH 6.8 [i.e. using 0.01N KH2PO4] and HPLC grade Acetonitrile [60:40]. Separation of the analyte was achieved using HPLC isocratic pump attached to the UV-VIS detectorC18, guard column and C18 column. The injection volume was 20 micro L, detected at 274 nm; flow rate: 1mL/min. Standard calibration curve was measured and found linear from 0.1 to 40 micro g/ml. The validation parameters were measured according to FDA guidelines and successful results were obtained. The presented analytical method could be employed for pharmacokinetic studies
ABSTRACT
A swift, precise and simple HPLC bioanalytical technique with UV detection was established and validated for quantitative estimation of valsartan in human plasma. The analyte was separated from plasma by protein precipitation with acetonitrile and chromatographically separated on Zorbax SB-C18 [5 micro m, 4.6mm x 15cm] column. The solvent mixture system consisting of acetonitrile, water and glacial acetic acid [40:59:1 v/v], was pumped using isocratic mode at 1mL/min flow rate. Samples' detection of drug was made spectrophotometrically at a wavelength of 264nm. The analyte response was instituted to be linear from 0.06 to 8 micro g/mL with a regression value of 0.999. The accuracy of the proposed method was ranged between 97.2-100.3% with 5% RSD. The analytical recovery [>95%] was consistently observed and satisfactory sample stability was also found at different environmental conditions. In conclusion the reported bio-analytical method is easy and robust that was successfully utilized in estimation of valsartan in a pharmacokinetic study
ABSTRACT
This assessment aims to determine the prevalence of methicillin resistance and multidrug resistance [MDR] among the clinical isolates of Staphylococcus aureus and antimicrobial susceptibility profile of methicillin resistant Staphylococcus aureus [MRSA] to the frequently prescribed antibiotics in Karachi. Isolates of MRSA, recovered from various clinical samples were included in this prospective, cross-sectional study from Jan 2015 to June 2017. Agar diffusion method was employed according to the protocols of Clinical Laboratory Standards Institute. Out of total 346 S.aureus strains, the frequency rate of MRSA was 52 % [n = 180]. MRSA infection was found higher among the age group 21-30 years i.e. 30% [n=54], followed by 20 % [n=36] in 31-40 years. Frequency of MRSA percentage in male and female was and 70 % and 30 % respectively. MRSA was more frequently observed in blood 20 % [n=36]. MRSA showed high resistance [100 %] to Oxacillin and Cefoxitin while 25% Vancomycin resistant S. aureus [VRSA] isolates and 25% Teicoplanin resistance were also reported. MRSA exhibited 16% resistance to Minocycline. It was concluded that MRSA pose a challenging threat to public health in Karachi. In addition, MDR should be periodically checked to avoid treatment failure
ABSTRACT
The objective of the present work was to develop Immediate Release [IR] tablets of Metoprolol Tartrate [MT] and to compare trial formulations to a reference product. Six formulations [F1-F6] were designed using central composite method and compared to a reference brand [A]. Two marketed products [brands B and C] were also evaluated. F1-F6 were prepared with Avicel PH101 [filler], Crospovidone [disintegrant] and Magnesium Stearate [lubricant] by direct compression. Pharmacopoeial and non-pharmacopoeial methods were used to assess their quality. Furthermore, drug profiles were characterized using model dependent and independent [f2] approaches. Brands B and C and F5 and F6 did not qualify the tests for content uniformity. Moreover, brand B did not meet weight variation criteria and brand C did not satisfy requirements for single point dissolution test. Of the trial formulations, F2 failed the test for uniformity in thickness while F4 did not disintegrate within time limit. Only F1 and F3 met all quality parameters and were subjected to accelerated stability testing without significant alterations in their physicochemical characteristics. Based on AIC and r2adjusted values obtained by applying various kinetic models, drug release was determined to most closely follow Hixson-Crowell cube root law. F1 was determined to be the optimized formulation